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ncKnight® NK Expansion Factor Kit
Cat. No. SN-03-0030
Features:Support clinical-grade/pharmaceutical-grade cell culture; Low blood volume requirement: 10-20 mL of peripheral blood is sufficient for one NK cell expansion; Initial cell requirements are low: supporting the initial Expansion of 5E6-2E7 mononuclear cells; High cell expansion rate: NK cells can expand 3000-6000 times within 13-15 days; High purity of NK cells: expanded NK cells can have a purity of 80%-95%.
Product Amount Cat.No. Quantity Storage
ncKnight® NK Expansion Factor Kit Contains: 1Kit(2L) SN-03-0030 1  

ncKnight® NK Expansion Factor Kit

NK Expansion Basal Medium

1L SN-03-0020 2

Store at 4°C

Ship at 2 ~ 8°C

ncKnight® NK Expansion Factor Kit

NK Expansion Factor A

270μL  SN-03-0031 1

Store at -20 ~ -80°C

Ship on dry ice

ncKnight® NK Expansion Factor Kit

NK Expansion Factor B

120μL  SN-03-0032 1
  • Overview
  • Citation
  • ncKnight® NK Expansion Factor Kit is an independently developed product by Shownin Biotech, designed for the expansion and culture of human natural killer (NK) cells. This defined, cytokine-only culture system enables efficient expansion of NK cells derived from human peripheral blood. When starting from PBMCs containing approximately 10% NK cells, a 3,000–6,000-fold expansion can be achieved within 13–15 days. The resulting NK cell population demonstrates high purity, with CD3⁻CD56⁺ expression levels exceeding 80–95%.

     

    The Morphology of NK Cell Expansion

     

     

    *All samples started with 10-20 million fresh/frozen PBMCs, using the ncKnight® NK Expansion Factor Kit. After 13-14 days of expansion in a 2L culture system, 5-7 billion NK cells can be harvested in a single collection.


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