| Product | Amount | Cat.No. | Quantity | Storage |
| ncKnight® NK Expansion Factor Kit Contains： | 1Kit | SN-03-0030 | 1 | |
|
ncKnight® NK Medium Basal Medium |
1000mL | SN-03-0022 | 2 |
2 ~ 8°C |
|
ncKnight® NK Medium Supplement |
40mL | SN-03-0021 | 2 |
-80 ~ -20°C |
|
ncKnight® NK Expansion Factor A |
270μL | SN-03-0031 | 1 | |
|
ncKnight® NK Expansion Factor B |
120μL | SN-03-0032 | 1 |
- Overview
- Citation
-
ncKnight® NK Expansion Factor Kit is an independently developed product by Shownin Biotech, designed for the expansion and culture of human natural killer (NK) cells. This defined, cytokine-only culture system enables efficient expansion of NK cells derived from human peripheral blood and umbilical cord blood. When starting from PBMCs containing approximately 10% NK cells, a 5,000–10,000-fold expansion can be achieved within 14–15 days. The resulting NK cell population demonstrates high purity, with CD3⁻CD56⁺ expression levels exceeding 80–95%.
The Morphology of NK Cell Expansion
*All samples started with 10-20 million fresh/frozen PBMCs, using the ncKnight® NK Expansion Factor Kit. After 13-14 days of expansion in a 2L culture system, 5-7 billion NK cells can be harvested in a single collection.
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