hPSC-MSC Differentiation Kit is crafted to facilitate the efficient differentiation of mesenchymal stem cells (MSCs) from human pluripotent stem cells (hPSC). This kit empowers researchers to seamlessly transform hPSC into high-purity MSCs, presenting stable proliferation, normal karyotype and express characteristic surface markers (CD73+/ CD90+/ CD105+, CD14-/ CD34-/ CD45-/ CD79α-/ HLA-DR-), and tri-lineage differentiation potential (osteogenesis, cartilage, and adipose differentiation). Therefore, hPSC-MSC Differentiation Kit can be applied in various in vitro experiments, drug screening and safety evaluation, as well as cell transplantation in disease animal model.

Process

Cell morphology and identification

A, B, C: cell morphology on days 3, 8 and 14 during the differentiation process of hPSC-MSC Differentiation Kit.

D, E, F: continuous culture of hPSC-MSC, showing the Day3 morphology of P1, P3, and P6 generation cells respectively. Scale bar: 200μm.

Cell surface marker detection

Surface marker detection of continuously cultured hPSC-MSC (P5): CD73+/CD90+/CD105+, CD14-/CD34-/CD45-/CD79α-/HLA-DR-

(The picture above shows the results of flow cytometry analysis, the light gray line is the alloantibody negative control, and the black line is the expression of MSC cell surface markers)