hPSC-Nephron Differentiation kit
Cat. No. RP01015
Features：Used for efficient differentiation of hPSC into renal organoids.
| Contents | Amount | Cat. No. | Storage |
| The hPSC-Nephron Differentiation Kit-contains： | 1Kit | RP01015 | |
| Nephron Differentiation Supplement A（100×） | 1 mL | RP01015-A | -80℃ or -20℃ |
| Nephron Differentiation Supplement B（100×） | 1mL | RP01015-B | -80℃ or -20℃ |
| Nephron Differentiation Supplement C（100×） | 1.5 mL | RP01015-C | -80℃ or -20℃ |
| Nephron Differentiation Supplement D（100×） | 1.5 mL | RP01015-D | -80℃ or -20℃ |
| Nephron Differentiation Medium E | 500 mL | RP01015-E |
|
- Overview
- Citation
-
hPSC-Nephron Differentiation Kit（RP01015）is designed facilitate the efficient differentiation of human pluripotent stem cells (hPSC) into Kidney Organoid, exhibiting a nephron-like structure upon maturation. These mature organoids express specific marker such as CDH1, LTL, PODXL, etc., making them a versatile choice for various in vitro experiments, drug screening and safety evaluation, and cell transplantation studies in disease animal models.
Process
Cell morphology
A, B, C: cell morphology on days 4, 6 and 8 during the differentiation process of hPSC-Nephron Differentiation Kit.
D, E: cell morphology on days 14 and 20 of 2D differentiation; F: Enlarged illustration of cell morphology on day 20 of 2D differentiation.
G: cell morphology on the 13th day of 3D differentiation; H, I: Illustration of the cell morphology on the 17th day of 3D differentiation. Scale bar: 200μm.
Detection of specific marker expression of hPSC-derived renal epithelial cells
Illustration of specific marker expression detection (immunofluorescence staining) of hPSC- Nephron (2D differentiation, Day 20).
PODXL, CDH1, LTL and PAX8 are specific markers for podocytes, distal tubules and proximal tubules respectively (inverted fluorescence microscope). Scale bar: 100μm.
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