| Contents | Amount | Cat. No. | Storage |
| NcMission™ hMSC Medium V3.0 Contains： | 500mL | RP02010 | |
| NcMission™ hMSC Medium V3.0 Basal Medium | 500mL | RP02010-01 | 2℃~8℃ |
| NcMission™ hMSC Medium V3.0 Supplement | 25mL | RP02010-02 | -20℃~-80℃ |
NcMission™ hMSC Medium V3.0 stands as a clinical-grade/pharmaceutical-grade medium tailored for human mesenchymal stem cells (hMSC). Cultured with this medium, hMSCs exhibit stable proliferation, along with normal expression of cell surface markers and retained three-lineage differentiation potential.
Primary Culture of hMSC
A, B, and C: Cell morphology on days 6, 9, and 12 of culture respectively. Scale bar: 200μm.
D and E: The number of clones and cells on day 12 of culture compared with Commercial A medium respectively.
F: F: Compared with Commercial A medium, MSC climbing time.
Subculture of hMSC
hMSC cells continuously cultured in NcMission™ hMSC Medium were seeded at a density of 6000/cm2 with culture medium 0.2 mL/cm2 in T25 for 3 days.
G, H, and I: Cell morphology on day 1, 2, and 3, respectively.
J: Cumulative population doubling of cells continuously passaged from P1 to P10;
K: Population doubling time of cells continuously passaged from P1 to P10;
F: P8D3 hMSC.
Scale bar: 200μm.
Cell Surface Marker
NcMission™ hMSC Medium continuous cultured hMSC cells (P5)
(The picture above shows the results of flow cytometry analysis, the light gray line is the isotype control, and the black line is the MSC cell surface marker)
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