| Contents | Amount | Cat. No. | Storage |
| ncMission® hMSC Medium Contains： | 500mL | RP02010 | |
| ncMission® Basal Medium V3.0 | 500mL | RP02010-01 | 2℃~8℃ |
| ncMission® Supplement V3.0 | 25mL | RP02010-02 | -20℃~-80℃ |
ncMission® hMSC Medium V3.0 stands as a clinical-grade/pharmaceutical-grade medium tailored for human mesenchymal stem cells (hMSC). Cultured with this medium, hMSCs exhibit stable proliferation, along with normal expression of cell surface markers and retained three-lineage differentiation potential.
Primary culture of hMSC
Illustration of isolating and culturing umbilical cord hMSC using ncMission hMSC Medium (0.5g umbilical cord Wharton's jelly tissue/T75 culture bottle).
A, B, and C: Cell morphology on days 6, 9, and 12 of culture respectively. Scale bar: 200μm.
D and E: The number of clones and cells on day 12 of culture compared with Commercial A medium respectively.
F: F: Compared with Commercial A medium, MSC climbing time.
Subculture of hMSC
hMSC cells continuously cultured in ncMission hMSC Medium were seeded at a density of 6000/cm2 with culture medium 0.2 mL/cm2 in T25 for 3 days. G, H, and I: cell morphology on day 1, 2, and 3, respectively.
J: Cumulative population doubling of cells continuously passaged from P1 to P10; K: population doubling time of cells continuously passaged from P1 to P10; F: P8D3 hMSC. Scale bar: 200μm.
Cell surface marker
ncMission hMSC Medium continuous cultured hMSC cells (P5):
(The picture above shows the results of flow cytometry analysis, the light gray line is the isotype control, and the black line is the MSC cell surface marker)
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