ncMission® M30 hMSC Medium
(Phenol Red-free)
ncMission M30 hMSC Medium (Phenol Red-free)
Cat. No. SN-02-0030
| Contents | Amount | Cat. No. | Storage |
|
|
525mL | SN-02-0030 | |
|
ncMission® M30 MSC Basal Medium (Phenol Red-free) |
525mL | SN-00-0003 | 2℃～8℃ |
|
ncMission® M30 MSC serum-free supplement |
25mL | SN-02-0031 | -20℃～-80℃ |
The complete ncMission M30 hMSC Medium can be stored at 2-8°C for up to 2 weeks.
- Overview
- Citation
-
ncMission®M30 MSC Expansion Medium stands as a clinical-grade/pharmaceutical-grade medium tailored for human mesenchymal stem cells (hMSC). Cultured with this medium, hMSCs exhibit stable proliferation, along with normal expression of cell surface markers and retained three-lineage differentiation potential.
ncMission®M30 MSC Expansion Medium (Phenol Red-Free) is a phenol red-free version of ncMission®hMSC Medium V3.0.
Isolation and Subculture of primary hMSC
Illustration of isolation and culture of umbilical cord-derived hMSC using ncMission® M30 hMSC Medium (Phenol Red-free) (0.5g umbilical cord Wharton's jelly tissue/T75 culture flask)
A, B, C: the Cell morphology on day 6, 9, and 12 after isolation and seeding the tissue, respectively. Scale bar: 200μm.
D: Under the same seeding density and culture time conditions, the average population doubling is 14.1 times and the average population doubling time is 18.9 hours from P1 to P5 in
ncMission®M30 hMSC Medium (Phenol Red-Free) culturing system.
E: from 5g of Wharton's jelly tissue isolated umbilical cord, it can obtain 2.1x10⁷ cells in P0 and obtain 8.6x10¹⁰ cells in P3 cultured in ncMission®M30 hMSC Medium (Phenol Red
Free) system , and the number of cells is calculated theoretically based on the growth curve;
According to the standard dosage of 5x10⁷ per dose, it is expected to produce over 1600 doses of the product;
F: Illustration of the MSC creep-out time in ncMission®M30 hMSC Medium (Phenol Red-Free) compared to Commericial A Medium.
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